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The initial response shows the current-voltage relationship recorded by two-electrode voltage clamp in an AQP1-expressing oocyte in isotonic 100 mM Na+ saline (see Methods).
Signals obtained by recording voltage changes occurring on skull surface as a result of electrical activity of active neurons in the brain are called EEG [3].
Despite this, I decided to verify this previous finding by recording voltage responses from mature apical and basal gerbil IHCs P29-P366) usinewnew perforated patch experiments.
For spike recording, voltage signals from four channels of a tetrode were digitally band-pass filtered between 600 Hz and 9 kHz.
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For recording voltage-gated channels in a giga-sealed whole-cell configuration a holding potential of – 100 mV was applied to the myotubes.
Functional confirmation of cell-specific BK ablation was obtained by recording voltage-activated K+ currents in IHCs (Fig. 3 ) at P18 P24, an age at which BK currents are fully expressed in control animals (4).
Cells were clamped at −80 mV and pulsed for 20 ms from −60 mV to +60 mV in 5 mV steps when recording voltage-gated Ca2+ currents or clamped at 0 mV and applying ramps from −100 mV to +100 mV (400 ms) at 0.2 Hz to record TRPM5 currents.
At the start of each recording session, oxonol voltage-sensitive dyes were infused over a period of 2 h.
(C ) Summary of the dendritic recordings, showing peak first temporal derivative of dendritically recorded voltage (dV/dt), normalized to the median value for Stim.
The maximum recorded voltage at an external resistance of 30 Ω was 861.27 mV.
The datalogger consists of an internal electric circuit, which records voltage (V) at a set interval.
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