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For example, I scheduled a weekly team report to deliver on Wednesday evening, when I was recording the background music to an opera aria.
After recording the background emission for 1 min, 100 nM (final concentration) of ThG was added to cell suspension and fluorescence was recorded for ∼5 min. The resulting kinetic curves were integrated using standard PTI software and normalized for ThG effect without the PABA/NO addition.
After recording the background [Ca2+]cyt level for 1 min, the response to the CWE was measured for 10 min. Roots which did not respond to the stimulus were used for the total discharge reaction to ensure that the lack of [Ca2+]cyt elevation is not caused by a mutation in the apoaequorin gene.
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His musical break came when he met the composer Dimitri Tiomkin while conducting the Johnson choir as it recorded the background score for the movie "Lost Horizon" (1937).
If you record the background scenery then you would lose the sense of depth and so if you look very carefully at the invisible skyscraper you would essentially just see the picture on the wall.
There are a few microphone pops, some overly energetic guitar strumming and on "I Can Only Be Me," a ballad Stevie Wonder wrote but never recorded, the background vocals (sung by Cassidy) briefly override her lead.
We recorded the background images at the same places where we do our dataset in Table 3.
While reluctant at first, Houston agreed and re-recorded the background vocals to the praise of both Griffith and Davis.
A related approach is to record the background activity from the microelectrode.
The GC outline was then placed in an adjacent area clear of cellular material to record the background fluorescent intensity.
Additional ROIs of the same size were placed on the E. coli lawn to record the background fluorescence levels for each image.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com