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Light was combined by a series of dichroic mirrors and focused onto a 5 mm diameter piece of opal diffusing glass (Edmund Optics Inc., York, UK) positioned <1 mm from the eye (contralateral to the recording probe for LGN recordings).
Once the recording probe was in position mice were dark adapted for 1 h, which also allowed neuronal activity to stabilise after probe insertion/repositioning.
After recording a set of responses at one location the recording probe was raised or lowered by 400 µm and, after 1 h dark adaptation, another set of responses recorded.
A 64-site silicon recording probe (8 shanks, 8 recording sites per shank, 200 µm lateral spacing between shanks, 20 µm vertical spacing between recording sites) was implanted into the dorsal right hippocampal CA1 region under isoflurane anesthesia.
Light stimuli (460 nm; half peak width: ±10 nm), were generated by a custom built LED based light source (Cairn Research Ltd ., passed through neutral density filters as necessary, and was focused onto a 5 mm diameter opal diffusing glass (Edmund Optics Inc., UK) positioned 3 mm from the eye contralateral to the recording probe.
A recording probe (A4X8-5 mm-50-200-413; Neuronexus, MI, USA) consisting of 4 shanks (spaced 200 µm), each with 8 recordings sites (spaced 50 µm) was then positioned centrally on the exposed skull surface, perpendicular to the midline, and lowered to a depth of 2.2 to 3.4 mm using a fluid filled micromanipulator (MO-10; Narishige).
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The authors also discuss the use of electrical recording probes: "There are technical hurdles to be surmounted, but when the technology is perfected, recording from many thousands of neurons is conceivable".
The correlation coefficient in recording probing depth was 0.86; calculus with plaque deposits, 0.89, and gingival recession, 0.91.
On the day of sampling, in-house recording probes (Lupinsky et al., 2010), were connected to a central swivel (Model 72-0000, Hollistonpparatus, Holliston, MA).
Tamura et al. reported that the median value of the correlation coefficients was 0.08 for pairs of closely located cells isolated from a single-shaft electrode with multiple recording probes.
The Affymetrix Expression Console was used to record probe cell intensity files (.CEL) and probe level summarization files (.CHP) generated.
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