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After rinsing, the biocytin-filled cells were coverslipped in PB and documented by recording image z-stacks under a fluorescence microscope.
The depth of field was improved by recording image stacks and combining the single images into one sharp photograph with the software Combine ZP [ 69].
Images of whole glands were generated by recording image stacks of 1.8 µm and extended-focus images were calculated (wavelets method, no alignment).
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When it comes to recording images, Eastman Kodak has met its match.
CCD = charge coupled device, for recording images.
The thermal camera is capable of recording images with a sampling rate of 1 10 Hz without visible image recording.
Moreover, recording images in constant height mode allows to investigate the interaction between tip and molecule with high spatial resolution.
After recording, images of the alexa-filled neuron were acquired using 2-photon microscopy for morphological analysis.
As mentioned above, we very briefly (few seconds) turned off the respirator while recording images.
The entire set had the potential of recording images and of their digital analysis.
If required even 3D reconstructions could have been produced for all six complexes by recording images of tilted specimens.
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