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(A ) Left, recording configuration.
(A ) Top, recording configuration.
(B ) Left, recording configuration.
Top, recording configuration.
(A ) Schematic of recording configuration.
Left: recording configuration showing the activated circuit.
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Data in Fig. 4a d were acquired by scanning a series of 108 rectangular recording configurations across the whole array, with 60 stimuli (4 Hz) sent per configuration.
Stimulation-triggered electrical images and movies were compiled by scanning ~100 recording configurations across the whole 1.8 × 2.0 mm2 array, whereas stimuli were applied at a single electrode multiple times per configuration.
A first step in an experiment is to identify the locations of the neurons growing over the array, which was done by scanning a sequence of recording configurations across the array.
(a) Time dependence of the first-order diffraction efficiency in (ss) and (sp) recording configurations in the Ag/TiO2 nanocomposite film; and (b) Competition growth of the two holographic gratings: the (ss) grating recording separately in the first 200 s, followed by the overlapping with the (sp) grating recorded from 200 s to 400 s.
This light source is routinely used to evoke spikes in ChR2-YFP-positive cells in our laboratory, but even long, 500 ms flashes never produced an action potential in ChR2-YFP-NavII-III-expressing cells (Figure 5A; on-cell and current-clamp I = 0 recording configurations, n = 5).
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