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Recordings were conducted in an electrically shielded recording chamber equipped with a Neuroscan EEG amplifier.
Here, we present a novel brain slice recording chamber design that accomplishes this goal.
Coverslips were placed in an RC-25F (Warner Instruments, Hamden, CT) 500 μl recording chamber at 34 36 °C.
Drug solutions, in gassed ACSF, were introduced through a stopcock arrangement to the recording chamber with a latency of ∼20s.
The coverslips were transferred to a recording chamber before use.
A recording chamber was surgically implanted in the left hemisphere of each monkey.
Slices were transferred to a submersion type of recording chamber.
Slices were transferred to an immersion recording chamber and perfused (2.5 mL/min) with ACSF.
Following 3 minutes of PTZ exposure, the recording chamber was evacuated and replenished with tank water.
EEG and EMG recording began following one-week acclimation to the tether and recording chamber.
A recording chamber was attached over the trephination with dental acrylic.
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