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Cells were washed with HBSS twice, and recording buffer (2.5 mM probenecid in HBSS) and various compounds were added.
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All chemical compounds were from Sigma Aldrich and dissolved directly into ND96 recording buffer to a final concentration of 10 mM unless otherwise noted.
Currents were measured in ND96 recording buffer containing (in mM): 96 NaCl, 2 KOH, 0.3 CaCl2, 1 MgCl2, 10 HEPES, pH 7.6.
In a subset of subjects (N = 6) the electrode placement slipped or a recording buffer error truncated data collection.
Similar(49)
The voltammograms recorded in buffer solution at the bare CPE exhibited no any voltammetric peak over the entire pH range (Fig. 3, curve C) and in diltiazem solution (curve B).
Absorbance at 335 nm was recorded taking buffer of the same composition (without sample) as a blank.
Therefore, the UV vis spectra of 0.8 g/L H40AP16 (a) and H40AP8PAA4 (b) microgels were recorded in buffer solutions in a pH range of 2.2 7.4.
For interaction studies proteins were prepared in buffer E (50 mM HEPES pH 7.5, 150 mM NaCl and 0.1% Tween 20) and all the measurements were recorded using buffer E at a flow rate of 30 µl/min.
The pH of samples was determined (model B-212 Horiba pH meter) and 13C-HSQC spectra were recorded in standard buffer only (control), standard buffer with non-labeled T87 extract, or in standard buffer with labeled T87 extract.
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