However, it also may have caused some workshop fatigue, particularly towards the end of the workshop where teams discussed and recorded solutions.
Slices were perfused with ACSF recording solution supplemented with 1 μM tetrodotoxin to prevent action potentials and 100 μM picrotoxin to suppress inhibitory currents35.
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After at least 20 min of stable recording, the recording solution was switched to recording solution containing 0.01% DMSO; 15 min later hippocampal slices were perfused with recording solution containing 0.01% DMSO and either E2 or G1 at 10 nM.
Menthol and icilin stocks were dissolved in DMSO and diluted into recording solution immediately prior to experiments.
A recycling perfusion system was used to minimize recording solution volumes (5 mL) and allow the continuous perfusion of the caged MNI-glutamate (1 mM).
Standard recording solution contained (in mM): 90 KCl, 3 MgCl2, 10 Hepes (pH 7.4).
The offset potential of the tip was routinely adjusted after immersion into recording solution.
External recording solution contained (in mM): 114 CsCl, 20 BaCl2, 1 MgCl2, 10 HEPES, 10 glucose, adjusted to pH 7.4 with CsOH.
The recording solution contained (in mmol/L) 90 NaCl, 1 KCl, 10 HEPES, 0.5 BaCl2, 0.01 EDTA (pH 7.4).
The recording solution contained (mM): 130 NaCl, 5 KCl, 8 d-glucose, 10 HEPES and 1.2 MgCl2, titrated to pH 7.4 with NaOH.
