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During data acquisition care had to be taken that all right ventricular structures were clearly visible in all recorded planes.
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The recorded plane was parallel to the surface, but not really parallel to the laminae.
The recorded plane covered a large part of field L centred on the L2 sub-area described in wild starlings [40].
Results show that, for the particular excitation/emission wavelengths considered (488 nm and 669 nm, respectively), the dimensions of the scattering volume during uniform illumination extend around 3 times further in the surface recording plane than in depth.
The proposed method is based on the possibility of the digital holography to perform whole reconstruction of the recorded wave front, so that phase and intensity distribution at a plane located between the object and recording plane and along the reconstructed image of the object can be determined.
In planes of recording electrodes parallel to the epicardium and endocardium, the initial isochrones were elliptical with the major axes of the ellipses along the mean fiber orientation between the pacing site and recording plane rather than along the local fiber orientation in the recording plane.
The recording plane was considered completed when no response was obtained in both outermost penetrations.
The anatomical locations of the recording sites were determined from reference marks consisting of Alcian blue injections made after the last recording session through a glass micropipette, at 4 defined locations within the recording plane of each hemisphere.
Each recording plane consisted of up to 10 penetrations systematically placed at regular intervals of about 220 µm in a rostrocaudal row, between 230 1090 and 2220 3080 µm from the bifurcation of the sagittal sinus.
Each recording plane consisted of 1 to 3 penetrations systematically placed at regular intervals of about 230 µm in a rostrocaudal row (see Fig. 6), between 100 905 and 2496 3245 µm from the bifurcation of the sagittal sinus.
Kemal Amarasingham recorded the planes' sound effects, which he said involved "risking his life" by standing near jet engines and under wings.
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