Cardiorespiratory parameters at a PEEP of 15 cmH2O were recorded on a Biopac system (Biopac System Inc. Goleta, CA, USA) [ 20] and a P V curve of the respiratory system at a PEEP of 15 cmH2O was measured with the low constant flow method (9 L/minute) [ 12].
Infrared spectra were recorded on a Schimadzu system 160 FT-IR spectrophotometer (Shimadzu Corporation, Kyoto, Japan) using KBr pellets.
Infrared spectra were recorded on a Shimadzu system FT-IR 8400S spectrophotometer (Shimadzu Corporation, Kyoto, Japan) using KBr pellets.
The bioactive fractions were recorded on a HPLC MS/MS system which consisted of the HPLC Premier XE (Waters, MA, USA) connected through a split to the mass spectrometer (Waters, MA, USA) detector system operating at 45 °C.
UV spectrum was recorded on a Mariner System 5304 Spectrometer.
After calibration, all pressures were recorded on a computer system.
The arterial pressure was recorded on a polygraph system (RM6000; Nihon Kohden, Tokyo, Japan).
Signals were recorded on a computerized system (Rembrandt; Rescare, Buffalo, NY).
Sleep was continuously recorded on a computerized system (Grass Technologies, West Warwick, Rhode Island, USA) scored in 30-sec epochs according to American Academy of Sleep Medicine (AASM) standardized criteria [ 10].
Immunofluorescence was observed and recorded on an imaging system comprising a Coolsnap HQ CCD camera (Photometrics Ltd ,Tucson, AZ) and Zeiss Axioplan II fluorescence microscope with Plan-neofluar objectives of a Nikon A1R confocal microscope.
