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The method is based on the electromagnetic time reversal theory and relies on the use of a single measurement point to record voltage transient signals originated by a direct lightning strike either on the phase conductors or shielding wires, and possible flashovers.
Each of these depth electrodes has 10 to 16 channels that record voltage signals from the neurons surrounding it.
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Conventional whole-cell recordings were performed in dissociated cells to record voltage-gated Na+ and K+ currents, spontaneous mPSCs and ligand-gated NMDA or AMPA evoked currents.
One µM TTX was added to record voltage-dependent K+ currents immediately after pulling nucleated patches.
The composition of the external saline solution used to record voltage-activated Ca2+ currents was (in mM): tetraethylammonium chloride (145), CaCl2 (10), glucose (5), HEPES (10), pH 7.4 (with TEAOH).
To record voltage-sensitive fluorescence, we used two 14 bit fast CCD cameras (SciMeasure Inc) with 80x80 pixels resolution.
The same bath solution was used to record voltage-gated calcium channels in the whole-cell/whole-cilium configuration.
In previous work, the intracellular solution used to record voltage-gated Ca2+ currents from muscle cells of S. mansoni was titrated with NaOH to bring the pH to a physiological value [ 41].
To record voltage-gated calcium currents we employed an external solution containing (in mM): 140 tetraethylammonium-Cl, 3 CsCl, 5 CaCl2, 1.2 MgCl2, 10 HEPES and 10 glucose (pH 7.4 adjusted with Tris); and an intracellular solution: 140 CsCl, 1 EGTA, 4 Na2ATP, 0,1 NandTP and 10 HEPES (pH 7.2 7.3 adjusted with Tris; and 295 300 mosmoles/l).
(C ) Summary of the dendritic recordings, showing peak first temporal derivative of dendritically recorded voltage (dV/dt), normalized to the median value for Stim.
The maximum recorded voltage at an external resistance of 30 Ω was 861.27 mV.
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