Compared to recordings from acute SCN slices with metal or glass extracellular electrodes which typically produce only one or two circadian cycles of activity before losing stability of the recording configuration [21] [24], multi-electrode array recordings of SFR can be used to determine the period from 2 successive activity peaks, providing an average SFR period value for each recording.
Furthermore, although Ih is known to be subject to rundown in whole-cell recordings and intracellular dialysis would have been rapid and pronounced in our recording configuration, we doubt that this contributed to the Ih activation range in our study.
The SOMA media is not preferred to be used in the conventional recording configuration.
In spite of that, the SASW configuration remains attractive due to the limited number of receivers, as well as, the Rayleigh modes contributing in SASW records configuration can be identified by multiple-filter technique and isolated using time-variable filters.
We illustrate the utility of this recording configuration by measuring current sources and sinks during theta burst stimuli that lead to the induction of long-term potentiation in hippocampal slices.
However, despite the high signal-to-noise ratio levels that can be achieved with this recording configuration, the clinical use of cuff electrodes in closed-loop controlled neuroprostheses remains limited.
Intercellular dye diffusion patterns were recorded by a cooled digital CCD camera (AxioCam, Carl Zeiss, Germany) at various time points after the establishment of whole cell recording configuration.
Alternatively, small peptides can produce minute disturbances in membrane stability causing a low resistance pathway under the membrane patch, known as a "perforated recording configuration" [19].
The whole-cell recording configuration from neurons expressing EGFP was achieved using an EPC-7 amplifier (HEKA, Germany) and a Digidata 1200 acquisition board (Axon Instruments).
Compared to the VC configuration, where INa characteristics are measured at fixed membrane potentials, in alternating VC/CC experiments the conditioning membrane potential is kept at a fixed (predetermined) value before the recording configuration is switched to the CC recording configuration.
