Sentence examples for reconstitution system from inspiring English sources
We now have identified Syk mutant that are altered in auto-inhibition and tested their activity in the S2 Schneider cell reconstitution system as well as in reconstituted Syk deficient B cell lines.
In order to investigate the role of ATG16L1 variants in autophagy, we applied a knock-down and reconstitution system using RNAi to ablate endogenous ATG16L1, while reconstituting ATG16L1 expression with RNAi-resistant cDNA constructs.
We developed an inducible reconstitution system to interrogate rhomboid proteolysis quantitatively within the membrane in real time.
This in vivo reconstitution system provides a new approach for examining ligand binding and G protein coupling to cell surface receptors.
These strains thus appear to be useful for biochemical and structural studies of the human β3AR in an in vivo reconstitution system.
Nutrient flow was calculated using the reconstitution system based on Cr, Yb, and indigestible neutral detergent fiber and using 15N as microbial marker.
To this end we established and applied a number of various reconstitution systems of the outer membrane reaching from monolayers at the air water interface, via solid supported symmetric and asymmetric bilayers, free-standing symmetric and asymmetric bilayers prepared according to Montal-Mueller technique to three-dimensional aggregates such as liposomes.
We integrated diverse analytical approaches including spectroscopy, defined reconstitution systems, and cleavage site mapping in living cells to probe the role of the membrane.
With over a dozen crystal structures and well-developed reconstitution systems for their study, arguably the best understood biochemically are rhomboid proteases (Urban, 2010).
Future study should be aimed to understand how osteoclast-specific molecules such as IRTKS and Tks5 regulate membrane fusion in in vitro reconstitution systems and in vivo.
A variety of naturally occurring and synthetic DNA sequences have been shown to be sufficient for positioning a nucleosome in purified in vitro reconstitution systems [ 4- 6].
