After ten weeks, reconstitution levels were evaluated by assessing Ly 5.2 and Ly 5.1 expression in blood and mice were considered reconstituted when hematopoietic cells were >80% donor-derived.
Thirdly, though expression of HLA-DR4 molecules in DRAG mice did not affect the reconstitution levels with human B cells as compared to control mice, the B cells developed by DRAG mice were functional and able to reconstitute serum levels of human IgM comparable to those in human sera, whereas the levels of human IgM in control mice were significantly lower (p = 0.0002).
The reconstitution levels obtained in the recipients repopulated with Gata3 −/− AGMs were also markedly improved.
Fibrinogen levels were no longer significantly different from baseline levels after baseline plasma reconstitution, though levels were still somewhat elevated compared to uncorrected levels, suggesting that reconstitution with the person's own plasma from baseline incompletely reduced the fibrinogen concentration toward baseline levels.
Compared to B cell reconstitution, the level of T cell reconstitution in the adult NSG mice injected with expanded CD34+ cells+ cells was fairly low (Figure 4M O, Table 2).
We also found that while the PHD mutant can be phosphorylated and activate downstream signals when overexpressed at high levels, low level reconstitution of knockout cells with the PHD mutant results in lack of MEKK1 phosphorylation after vinblastine treatment.
Furthermore, reconstitution of glutathione levels in BSO-treated 3T3-L1 cells by providing glutathione-ethylester was sufficient to improve the adipogenic capacity (Figure 3B).
All forms of treatment resulted in comparable high-level reconstitution of splenic and medullary haematopoiesis and no incorporation of donor cells into arterial endothelium.
Moreover, IS analysis is useful to address clonal diversity during hematopoietic reconstitution and the levels of HSPC marking and activity after transplant, thus providing readouts for efficacy.
The level of reconstitution in scid mice was however low (<1%) and consisted mainly of human T cells, as very few human B cells were able to survive.
