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By reconstituting in vitro severing reactions, we show that phosphomimetic modification at amino acid position 131 in Xenopus laevis p60 decreases severing and microtubule-stimulated ATPase activity without affecting the binding affinity of p60 for microtubules.
Our goal was to develop adaptable approaches for reconstituting in yeast protease activation pathways and networks, using Caspases as a prototype.
Monolayers of choroidal epithelial cells were cultured in bicameral devices, reconstituting in vitro the barrier properties of the blood-CSF interface.
IFNβ, IL-1, and IL-6 standards were freshly prepared by reconstituting in the standard diluent buffer to give a stock concentration of 400 pg/ml.
The dried FLAG-Rnd3 immunoprecipitation eluates were neutralized by reconstituting in 10 μl 1 M triethylammonium bicarbonate (TEAB) and drying in vacuo twice.
NK cells reconstituting in patients after umbilical cord blood transplantation mature more rapidly in patients infected with cytomegalovirus [32], a pattern also seen in primary HIV infection [33].
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Dried mushrooms are reconstituted in hot water.
(My dream is to see the company reconstituted in Brooklyn).
People have seemed thrilled to see their favorite works broken down and reconstituted in odd ways.
The original Scott Trust was formed in 1936, and reconstituted in 1948.
The real question is whether the army could be reconstituted in anything like its traditional form.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com