In addition, bone marrow from older humans is less efficient at reconstituting recipients when compared to the reconstitution capacity of bone marrow derived from younger patients [ 49].
These cells are drug resistant, express stem cell associated genes, and have high capacity for reconstituting tumors in vivo [ 9, 28, 30, 31].
When extensively T-cell-depleted marrow or mobilized blood was given, a low percentage (17%) of mixed chimerism could be detected at the stromal cell level, suggesting a limited but effective capacity of reconstituting the BM microenvironment [ 4].
Compared with known memory populations, TSCM have shown increased proliferative capacity, greater efficiency in reconstituting immunodeficient hosts, and superior anti-tumor activities in animal models.
Moreover, the BM cells from MLL/AF9 leukemic mice treated with DZNep had impaired capacity to reconstitute leukemia in vivo and attenuated colony forming capacity in vitro (Fig. 1f,g, Fig. S3).
These blood-forming stem cells are rare perhaps one in a hundred thousand cells and are unremarkable when seen under the microscope: small, bland, and round, offering no indication of their marvellous capacity to reconstitute the entirety of our blood system.
These blood-forming stem cells are rare — perhaps one in a hundred thousand cells — and are unremarkable when seen under the microscope: small, bland, and round, offering no indication of their marvellous capacity to reconstitute the entirety of our blood system.
By contrast, longevity, robust proliferative potential and the capacity to reconstitute the wide-ranging diversity of the T cell compartment are more critical for cells with permanent gene modifications.
