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Recently, mouse tooth germs reconstituted with the help of three-dimensional organ culture technologies (Ikeda et al. 2009; Nakao et al. 2007) or bioengineered tooth unit (Oshima et al. 2011) transplanted into the alveolar bone in the lost tooth region in an adult mouse yield tooth organ structures resembling those of native tooth.
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Dried samples were reconstituted with the mobile phase and.
With the help of Santa Barbara, Calif.
The residue was finally reconstituted with 200 μL of the desorbing solvent.
The extract was reconstituted with 5 uL of water and 5 uL of the unaggregated colloidal solution.
50 μL of the residue, reconstituted with 1 mL of deionized water, were injected.
Finally, the extract was reconstituted with 1 mL of methanol before HPLC analysis.
The samples were reconstituted with a buffer of a defined pH and incubated at 40 °C.
The apo-Pa5106 was reconstituted with 1.0 equiv of Zn2+ in 50 mM HEPES, pH 7.5.
The dry matter was reconstituted with 1 mL of MeCN/H2O (3/7, v/v) and spiked 0.5 mL of MeCN saturated n-hexane.
Finally, the concentrated protein was reconstituted with 500 μl of 0.15 M PBS, pH 7.2.
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