By mid-April, 2015, the memorial had been removed and reconstituted four times.
Using microbial genome information, we reconstituted three enzymes, PCD, DPR and GDH, for pterin regeneration, and coexpressed the corresponding genes in E. coli.
In order to reconstitute two independent DNA duplex strands, all domains of life have evolved a collection of structure-selective endonucleases which cleave DNA joint molecules with distinct substrate specificity (Table 1).
The methyltransferase SET domain of Ezh2 was located by reconstituting two complexes, one bearing an internal GFP tag immediately upstream of this domain Ezh2-GFP5955, Figure 9A, panel IV; see also Figure 9 figure supplement 1, Movie 1) and another with a C-terminal GFP tag (Ezh2-GFP, Figure 9A, panel V).
Here, we reconstitute four stages of nucleosome architecture using purified components: yeast genomic DNA, histones, sequence-specific Abf1/Reb1, and remodelers RSC, ISW2, INO80, and ISW1a.
It marks the first time an institution or individual has tried to reconstitute one of Francisco Goya's sketchbooks, which were broken up in 1826 after his death.
The AGE3-reconstituted strain UZ55 was generated by integration of plasmid pCaAge3-Sat2 (Table S2), which had been linearized before by Bsp1407I digestion, at the native AGE3 promoter of the age3Δ strain, thereby reconstituting one wild-type AGE3 gene copy.
The drug product samples were prepared by reconstituting ten drug product vials with normal saline to obtain a final concentration of 1 mg/mL based on label claim.
The mGRASP technique is based on fluorescence that is reconstituted when two non-fluorescent split GFP fragments targeted to the synaptic membranes in two separate neuronal populations are closely opposed across the synaptic cleft; thus, fluorescence uniquely indicates a synapse.
