He later conducted his experiments by using a cell-free system, in which the molecules thought to be important in vesicle transport were added to a test tube in an attempt to reconstitute the process and thereby eliminate certain complexities associated with the cellular environment.
However, we noted that GsbN expression in NB 6-4 was not able to completely reconstitute the process, since ACs and PCs were not always properly separated (Figure 6B3 and 6B4, compared to wild-type shown on Figure 6B1).
We seek to reconstitute the entire process from promoter chromatin remodeling to transcript synthesis with pure proteins and nucleic acids, to solve the structures of the proteins, and to elucidate their functional interactions.
However, to completely define the targeting membrane for IL-1β translocation, it is important to identify the translocon, or an equivalent, directly responsible for IL-1β translocation, as well as to reconstitute the translocation process in vitro.
To better understand the interplay of the E3/E4 ubiquitin ligases UFD-2 and CHN-1 in this process, we reconstituted the ubiquitination reaction in vitro using recombinantly expressed and purified components of the C. elegans system including the relevant E2 enzyme, UBC-215.
Xenoliths are usually reconstituted through the processes of contact metamorphism, in which heat and fluids cause mineralogic and chemical changes in the parent rock of the xenolith; a study of these changes can give information on the temperature and composition of the magmatic body.
We found that the AECs reconstituted the basement membrane that had been disrupted by the decellularization process.
In the late nineteen-eighties, it came up with the phrase "not from concentrate" to distinguish its pasteurized orange juice from the cheaper "from concentrate" or "reconstituted". The idea was to convince consumers that Tropicana's juice was a fresher, less processed product than reconstitute, and therefore worth paying more for.
The University never reconstituted the board.
The curators in Washington have reconstituted the arrangement handsomely.
