Sentence examples for reconstituted the expression from inspiring English sources
To exclude that the absence of EGFR transactivation by TGF- β could be due to a defect acquired during development of the Cav1 knockout mice and not to the mere absence of Cav1, we reconstituted the expression of Cav1 in Cav1−/− cells.
Importantly, we demonstrated that silencing of PC4 suppressed NHEJ by downregulating the expression of XLF in ESCC cells, whereas reconstituting the expression of XLF protein in the PC4-knockdown ESCC cells restored NHEJ activity and radioresistance.
More recently, a striking demonstration of its potential has been to use it to reconstitute the expression of the multisubunit T-cell receptor CD3 complex in bone marrow-derived stem cells transduced with retroviral vectors carrying up to four CD3 genes separated by 2A sequences [ 10, 11].
As illustrated in Fig 1A, in reconstituted mice the expression of AE and the GFP co-reporter gene can be regulated by administering doxycycline (DOX) [for review see (Bockamp et al, 2002; Bockamp et al, 2008)].
Prior work showed that POSX or POSY could be added to POS9 to reconstitute the INO expression pattern [ 12].
In preliminary experiments, we reconstituted the tetrameric PRC2 complex (Ezh2/EED/Suz12/RbAp48) in an insect cell expression system, following previous protocols established to reconstitute a functional PRC2 complex (Pasini et al., 2004; Ketel et al., 2005; Margueron et al., 2008).
Expression of recombinant LSR in LSR−/− HAP1 cells reconstituted the toxicity.
Microsoft has reconstituted the parts of that it can control.
Next we reconstituted the translocation in vitro.
As the source of regulatory elements to reconstitute the endogenous CCR1 expression, we used a BAC clone spanning 8 kb upstream and 34 kb downstream of the mouse Ccr1 gene (Fig. 1a).
Further, multiple copies of the POS9 element did not reconstitute the normal level of expression because detection of reporter activity required an elevated substrate concentration.
