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The reconstituted population behaved as the original population, with, however, some delayed lesion development.
Cooperativeness between PSPOS and PSNEG forms was definitely demonstrated by the in vivo evidence that none of the purified populations was able to progress and generate a lesion in susceptible (Fig. 5) and semi-resistant mice (Fig. S2); only a reconstituted population was capable of inducing lesions similar to those obtained with the original metacyclic population.
1, 26 However, reductions in new inflammatory disease activity following B-cell depletion in RRMS patients may be sustained in some patients even as the circulating B-cell count returns to normal, 31 suggesting a reconstituted population with a higher proportion of anti-inflammatory Breg-cells.
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In hematopoietic stem cells, all cells expressed the Sca1 surface marker at varying levels and the sorted low- and high-expressing cells reconstituted a population with the initial distribution slowly [2].
The reconstituted BM populations were analyzed for 6 weeks following transplantation.
To show that this same type of cooperativeness also happens in vivo, we infected BALB/c mice in the hind-foot pad with 105 parasites of the original metacyclic population, with the purified PSPOS and PSNEG sub-populations, and with a population reconstituted with a 1∶1 mixture of the two previous populations, and followed lesion size in individual mice.
Since most cancer cells are genetically unstable at either chromosomal or nucleotide level, cancer cells that recur after initial chemotherapy usually acquire drug resistance by accumulating genetic changes which lead to a selective advantage that allows them to survive the chemotherapy and reconstitute the population of tumor cells.
Additionally, neither Sca-1 (stem cell antigen) expression nor the SP phenotype was found to be expressed in the mammary reconstituting Lin-CD29hiCD24+cell Lin-CD29hiCD24+cell
Thus, the simple extrapolation of the animal data into future therapeutic strategies that aim at reconstituting this population does not seem to hold true in human organ-specific autoimmune diseases.
Transplantation of syngenic bone marrow into the irradiated mice reconstituted the leukocyte population in the blood and the macrophage and eosinophil density around the TEBs, and completely rescued ductal outgrowth (Fig. 2c,2d).
In vivo regulatory properties resided in the CD62L+CD25+CD4+ population, regardless of their previous culture with (n = 11, MST >100 days, p<0.0001 vs. MR; CD4pres) or without anti-CD4 (n = 3, MST >100 days, p<0.001 vs. MR, CD4abs), as seen by long-term graft survival in all mice reconstituted with this population and CD45RBhighCD4+ cells.
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