These reconstituted images all had the same frequency spectrum and differed only in phase.
All imaging row data were transferred to a Centricity RA1000 workstation (GE Healthcare UK, Ltd ,Buckinghamshire, UK), whereby 3D CT images, including reconstituted CT images, were created.
Reconstituted images corresponding to each of these three topographic fractions were pseudo-colored according to their labeled (immunofluorescent) TMP or fibronectin intensities, which ranged between 0 (the lowest) to 225 (the highest).
Cell-containing areas were selected by manually circumventing around cell clumps detected by cell nuclei (DAPI) staining using original reconstituted projection image of the entire 3D culture in each field and designated as 'cell region'.
ON and OFF times can be assessed by performing in vitro single-molecule analysis [ 21] but we chose instead to focus on the end result - the reconstituted STORM image - as an indirect measure of the blinking properties as well as the density (and therefore circumvent issues with translating in vitro measures).
When reconstituted projection images of the 3D cultures were produced to represent the topographical top, middle, and bottom layers as shown in Figure 5I L, the biggest difference induced by the two cell types to the matrices was observed again in the 'top' layers of the matrix.
In order to compensate the very weak expression levels, images were reconstituted averaging 100 images of a temporal sequence therefore minimizing the noise.
The Flexible spectral Imaging Color Enhancement (FICE) system estimates the spectrum reflectance from a white-light endoscopic image and reconstitutes a color image from the wavelength, including useful information about the GI tract.
The Times pushed the Post out of the arrangement ten years ago, making it inevitable that the Trib, if it survived, would eventually be reconstituted in the image of its sole owner.
In brief, this technology takes an ordinary endoscopic image and arithmetically processes the reflected photons for three different wavelengths (red, green and blue) to reconstitute virtual images.
A cluster of virtualization servers can be configured so that if any one server should fail, the others can reconstitute the images they were responsible for and carry on their work.
