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The natural cross-linking pathway of collagen does not occur in vitro and consequently reconstituted forms of collagen lack sufficient strength.
In vitro reconstituted forms of topoisomerase with only the NH2-terminal domain deleted (topo70) or both the NH2-terminal and the linker domains deleted (topo58/6.3), maintain enzymatic activity [13].
The metal ions were completely removed, at 25°C, to prepare the demetallated (apo) form, according to previously published protocols [29], and the zinc reconstituted forms were prepared, as well, as previously described [15].
Yet, reconstituted forms of BsQDO with Fe(III), Mn II), Co II), Ni II) or Cu II) as a cofactor showed similar or higher activities compared to their Fe(II) containing counterpart [114].
When I saw Walker's sculpture, on a brutally hot summer day, I wondered whether the artist had erred in imagining that her art would stay put in the space of the gallery, and not circulate in remixed and reconstituted forms.
Similar(54)
This reconstituted form of the enzyme, formed from separately purified polypeptides, allowed us to examine a detailed hypothesis for regulation of the ATase that resulted from prior studies.
This reconstituted form of the enzyme allowed us to investigate various aspects of the hypothesis for ATase regulation by glutamine, PII, and PII-UMP.
The drug product is provided as a mannitol boronic ester, Fig. 1b, which, in reconstituted form, consists of the mannitol ester in equilibrium with its hydrolysis product, the monomeric boronic acid (2).
We describe a reconstituted form of the ATase protein that differs from the normal wild-type enzyme by the lack of the peptide bond normally found between amino acid residues 608 and 609 in the 946-amino acid ATase.
The uniqueness of this new stratagem is that due to its peculiar fusion of suffering, sacrificial love, death, and universal redemption it was able to reinvigorate and reconstitute forms once spent.
With this, we estimated that the reconstituted native forms of H1.1 and swapped H1.1 were approximately 17% for both of them, as their spectra in that region are completely overlapping.
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