The Danio rerio full length VDR clone was reconstituted by PCR using overlapping 5' and 3' regions as templates.
A binding stoichiometry of 1 1 for IsdB-N2 and IsdH-N3 was estimated from reconstituted samples by using MCD.
Further, we determined that the intact TGF-β signal pathway was fully restored in AsPC-1 and CFPAC-1 stable SMAD4 reconstituted cells by using a SBE4 luciferase reporter assay, and by detecting the levels of SMAD2 phosphorylation after TGF-β1 treatment in AsPC-1 cells after SMAD4 restoration.
E. coli lyfo-disks were reconstituted by crushing one pellet using a sterile spatula in 0.5 mL of sterile TSB.
The number of fluorophore molecules per reconstituted receptor was calculated by using the maximum absorbance of the donor- or acceptor-labelled receptor.
Nucleosomes were reconstituted by the salt dialysis method [103] using HeLa core histones.
By using NPCs reconstituted from Xenopus laevis egg extracts, we show that Nup98 is essential for maintaining the permeability barrier.
The present study is the first direct demonstration of lipid translocation mediated by MsbA, and avoids the ambiguity of whole-cell or membrane systems, where multiple proteins are present, by using purified reconstituted MsbA.
Digestion solutions were concentrated to 20 μL using vacuum centrifugation and reconstituted by adding 40 μL 1% formic acid.
To probe the mechanism and selectivity of diolide formation, we sought to reconstitute ring formation in vitro by using a non-natural substrate.
