Sentence examples for reconstituted by combining from inspiring English sources

Moreover, the enhancement of pMMO propylene epoxidation activity by the presence of MDH could not be reconstituted by combining purified pMMO and MDH, and formation of a complex between purified pMMO and MDH has not been reported.

Despite the biolayer interferometry data, we were not able to isolate a stable pMMO MDH complex by SEC, consistent with previous studies in which the complex and its activity could not be reconstituted by combining purified proteins.

The TmPurLQS complex was reconstituted by combining the three proteins in a 1 1 2 ratio of TmPurL TmPurQ TmPurS, respectively, in their purification buffers, followed by addition of 5 mM ATP and 5 mM ADP.

Signaling complexes were reconstituted by combining 6.7 μM Tsr receptor, 5 μM CheA kinase, and 10 μM CheW adaptor protein in activity buffer [160 mM NaCl, 6 mM MgCl2, 50 mM Tris, and 3 mM EDTA (pH 7.5)] for 45 min at 22 °C in the presence of 0.5 mg/mL BSA, 2 mM TCEP, and 2 mM PMSF.

Functional, membrane-bound core complexes were reconstituted by combining 6.7 μM Tsr receptor, 5 μM CheA kinase, and 10 μM CheW adaptor protein in kinase assay buffer [160 mM NaCl, 5 mM MgCl2, 50 mM Tris, 0.5 mM ethylenediaminetetraacetic acid (EDTA), pH 7.5] for 45 min at 22 °C in the presence of 0.5 mg/mL BSA, 2 mM TCEP, and 2 mM PMSF unless otherwise specified.

The receptor kinase adaptor core units, as well as larger assemblies of core units ranging in size up to small hexagonal arrays, can be reconstituted in vitro by combining isolated, receptor-containing bacterial membranes with purified kinase and adaptor protein.

Here, we define the oligomeric state of full-length Get1/2 in reconstituted lipid bilayers by combining single-molecule and bulk fluorescence measurements with quantitative in vitro insertion analysis.

Figure 4B shows that none of the pairwise combinations had DNA binding activity; however, it was possible to reconstitute the Elba shift by combining all three translation products.

In the present study, we reconstituted a membrane system enriched in glycosyltransferases obtained by combining the properties of the wheat germ lectin with the dialysable detergent n-octylglucoside.

In the nineties, the composer most likely to be absorbed, quoted, and reconstituted by pop musicians was Ennio Morricone.

"We will get the board reconstituted by 8pm this evening and have a board meeting soon after that.