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We reconstituted USP15-KO cells with ectopically expressed WT USP15 or USP15 mutantD967H mutant.
We reconstituted USP15-KO cells with WT USP15 or a catalytically inactive mutant of USP15 (USP15-C269A).
B.T.D. and R.K.S. provided ApoA1 and Gs protein, and reconstituted β2AR in HDL particles with Gs.
The exposure to ultra-violet (UV) radiation was done in ISO reconstituted water71.
Suppressive activity of CD4+ T cells was studied by adoptive transfer.Titers of anti-GPI antibodies in reconstituted B6.g7 mice were ∼60-fold lower than in reconstituted B6.g7 Rag mice.
The data provide an effective mechanism for the production of resilient purified, reconstituted ABCB1.
However, long-term stability of purified reconstituted ABCB1 can only be reliably achieved with storage at −80 °C.
In addition, we reconstituted PARP2-deficient cells with full length PARP2 or the E545-to-A mutant (E545A), which disrupts the catalytic cage23.
When a reconstituted 36-year-old band headlines Siren, that tells you something (good) about the band and (not-so-good) about the festival.
To examine how USP15 phosphorylation affects cellular sensitivity to DNA damage, we reconstituted USP15-KO cells with ectopically expressed WT USP15 or USP15 S678A mutant.
To further characterize the recruitment of USP15 to DSBs by MDC1, we reconstituted MDC1-deficient cells with either FHA deletion mutant or BRCT deletion mutant.
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