DOI: http://dx.doi.org/10.7554/eLife.00792.013 To evaluate the contribution of the secretin YscC to the elasticity, we expressed and purified full-length YscC, reconstituted it into liposomes, and reconstructed its structure at a resolution of ∼3 nm by cryo-ET and sub-volume averaging.
In recent years, several companies have developed lines of book-quality recycled paper, which is generally made by bleaching the ink out of once-virgin fiber and reconstituting it into new paper.
We expressed VDAC-S193E and reconstituted it into DMPC lipid bilayers.
By site specifically labelling purified monomeric β2AR and reconstituting it into lipid vesicles, we are able to show that this receptor effectively forms specific multimeric assemblies in lipid bilayers as monitored by FRET and cross-linking studies.
It loves to take a uniform, chop it up and reconstitute it, like ham, into something new.
It is the first step to liquidating the company and reconstituting its assets into a new business.
In a flash of futuristic neon dance-powered magic, it reconstitutes itself into a shining black CD with one word printed on it: Control.
The SoPIP2 1 structure is more sensitive to the temperature changes when it is reconstituted into detergent micelles than when it is reconstituted into lipid membrane systems.
To avoid such precipitations, it was dissolved as soon as it was reconstituted, and divided into small aliquots.
Eileen Toback, executive director of the New York Professional Nurses Union, said she had hoped the staff would reconstitute into a new organization as soon as she heard the news.
KcsA was purified as described by Marius et al. It was reconstituted into lipid bilayers by mixing lipid and KcsA in cholate at a DOPC KcsA tetramer molar ratio of 40 1, followed by dilution into buffer [20 mM Hepes and 100 mM KCl (pH 7.2)] to decrease the concentration of cholate below its critical micelle concentration and to re-form membranes.
