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Thus, physiological analysis of these and other mtSSB mutants, in addition to development of new in vitro assays that reconstitute fully the mtDNA replisome, will be informative in understanding the mechanism of mtDNA replication.
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These promising preclinical results suggest that harvesting and grafting of microcolumns may be useful for reconstituting fully functional skin in human wounds, without donor site morbidity.
We report a novel process for reconstituting fully operational protein complexes directly from cellular membranes into nanoscale lipid bilayers using the SMA copolymer.
In the mouse, a single cell with the Lin-/CD24+/CD29high Lin-/CD24+/CD29high 31, 32] cantigeniceconstitute a fully functional mammary gland.
During the late phase (from ∼48 hpa onwards), the tail and its tissues (including blood vessels, neurons, and muscle) regenerate to reconstitute a fully functional appendage (Fig. 1E).
For the CD type, in most cases (230 over 252), the gene encoding the CD was colocalized with that of encoding the corresponding TAD (supplementary tables S2 and S3, Supplementary Material online), consistent with the notion that the presence of both are necessary to reconstitute a fully functional enzyme.
Strikingly the minimal combined truncation reconstituted LdTopIΔ548ΔL/ΔS fully conserved the sensitivity to CPT.
KO, D/A- and WT-reconstituted cells fully differentiated into mature adipocytes with KO and D/A cells exhibiting a trend for enhanced differentiation.
Heterologous co-expression of LdTopIL and LdTopIS genes in a topoisomerase I deficient yeast strain, reconstitutes a fully functional enzyme LdTopIL/S which can be used for structural studies.
C O bond cleavage mediated by Co2+ affords an 18-fold increase in fluorescence by releasing the ligand and reconstituting the fully conjugated fluorescein fluorophore.
To fully reconstitute the iron-sulfur clusters in RtelN, renatured protein was reconstituted with excess iron and sulfide as described previously [ 23].
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