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In the cophylogenetic analysis, we compared AVRk1 and TE1a trees, using reconciliation analysis with Jungles [34] as implemented in the program TreeMap 2.0β.
Results obtained in the reconciliation analysis with Jane show significant level of co-speciation in the Mimus- Analges association (P < 0.03, in both permutation tests).
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In order to elucidate evolutionary footprints, reconciliation analysis was performed with a species tree and an Argonaute gene tree.
In order to investigate evolutionary history of the genes in detail, reconciliation analysis was performed with the selected genomes (Table 1).
We also address incongruence among the gene trees with gene tree reconciliation analysis, and assign estimated ages to the nodes of our tree.
In the tree reconciliation analysis, the steep decrease in inferred ancient duplications with increasing strictness in duplicate retention likely reflects such gene loss.
To investigate gene duplication and loss events, reconciliation analysis was conducted by using Notung software (version 2.6) [ 60] with the species and gene trees.
For an event-based reconciliation analysis, we used JANE [ 63].
Our gene tree – species tree reconciliation analysis has verified the duplication pattern amongst the MHPs.
These clades (yellow boxes) group with the N-terminal modules of both lineages (Fig. 3, red boxes), with higher Bayesian (pp = 1.00) than ML (bs = 51%) support; a duplication at node 4 was inferred by the reconciliation analysis.
Furthermore, traditional reconciliation analysis is only as good as the supplied trees.
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