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Indeed, the standard Roche cDNA library preparation protocol recommends to start with 200 ng of RNA depleted of rRNA.
The American Urological Association (AUA) PSA Best Practice Statement Update 2009 recommends to start regular screening for prostate cancer at the age of 40 years in patients with an anticipated lifespan of more than 10 years [ 9].
Professor Clay Shirky recommends to start communication face-to-face and then move on to email.
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"It is really recommended to start thinking of the month of August as the transition, or at least when camp or other structured activities stop," Dr. Hoffman told me.
Most authors recommend to start preventive therapy two days before the onset of bleeding [10].
Thus, it is not recommended to start with a simple capsule formulation as service dosage form and to change later to a market ready tablet formulation.
Based on the model results it is recommended to start transport services between Hull and a Lower Rhine inland port (i.e. Dormagen).
It can be recommended to start using simple and easy to implement strategies of compliance and resource efficiency and later move towards more complex indicators, addressing environmental and social effects, supply chain, and lifecycle impacts [23].
Later in your career, if you want to learn about machine learning, I would recommend to start with a project: learn your way by trying to solve a problem that you have at heart.
Interested newbies are recommended to start with one of their Rosie packages, which include three hens, a locally built coop (choose from a variety of colors), two months of supplies, and Victory's basic 101 training.
Whether barbecue chips, cigarettes or diet soda, these are not the kinds of food groups recommended to start our day -- food groups that can give us a positive (and healthy) outlook.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com