The CPE was dissolved in DMSO, and control mice were treated with DMSO, which is the recommended solvent for water-soluble materials in the LLNA (Ryan et al. 2002).
Because the cortisol was available in water-soluble form (encapsulated with cyclodextrin), it was unnecessary to dissolve it in any solvent; however, the corticosterone was dissolved in the recommended solvent, methanol.
Check with the manufacturer of that oil to see if there is a recommended solvent for removing it that will not damage the deck material it has defaced.
And there is a number of recommended solvents for the latter, namely, water, methanol, ethanol, acetic acid, acetone, acetonitrile, DMSO, DMPA, nitromethane, and sulpholane [18].
Wy14643 (Sigma Aldrich) and recombinant human IGF-1 (Life Technologies) were dissolved in the recommended solvents.
Phenylephrine (PE), acetylcholine (Ach), NG-nitro-L-arginine methyl ester (L-NAME), sodium nitroprusside (SNP), phosphatidylinositide (PI 3-kinase inhibitor LY294002, and PI 3-kinasease B (Akt) inhibitor V were purchased from Sigma-ALY294002St. Louis, MO, USandand dissolved in recommended solvents.
Therefore, ethanol was recommended as the solvent for preparation of DPPH and analyte solutions.
For subsequent experiments, peptides were synthesized at a purity of 90% and suspended to 2.25 mM using the solvent recommended by the manufacturer.
Check with the supplier of the deck material to see if there is agreement for the use of the solvent recommended by the cooking oil manufacturer.
In support, OECD guidelines recommend that solvents should be used at a concentration below 0.1 mL L−1 (0.01%), which provides a reasonable working concentration for most solvents (OECD, 2000), and minimising concentrations was further supported by Hutchinson et al. (2006) after a review of evidence for the effects of solvents on ecotoxicological test species.
Drugs used in this study were dissolved in solvents recommended by the manufacturer.
