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Fluorescence intensity data was obtained with Agilent Feature Extraction Software v. 10.7.3.1, using recommended protocol GE1_107_Sep09.
The completed library was sequenced on an Ilumina Miseq platform according to the Illumina recommended protocol.
Cell surface exposed phosphatidylserine was evaluated using Annexin-V FITC conjugate (Invitrogen) following the manufacturer´s recommended protocol.
Cells were transfected in quadruplicates using the Lipofectamine LTX Plus Reagent (Invitrogen) according to the manufacturer's recommended protocol.
Brain: whole brains were incubated in CellEvent Caspase 3/7 Green (Thermo Fisher) according to the recommended protocol.
When a recommended protocol cannot be implemented in full, a selection of tests may be adequate.
PCR was performed using PrimeSTAR HS polymerase (Takara) following the manufacturers recommended protocol.
according to the manufacturer's recommended protocol.
Assays were performed according to the manufacturer's recommended protocol.
The assay was performed according to the manufacturer's recommended protocol.
The Qiagen recommended protocol for FFPE tissue was then followed.
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