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A pot experiment was conducted to assess the efficacy of six selective herbicide treatments applied at three rates (recommended (medium) rate, half (low) rate and double (high) rate) for controlling broom of various ages (3, 6, 9 and 12 months old).
NHDF cells cultured in recommended medium were also effectively inhibited by the addition of the anti-WNT10A antibody (Figure 4D).
All cell lines were cultured in recommended medium in the presence of 10% FBS in a humidified atmosphere containing 5% CO2 at 37°C.
LS174 human colon adenocarcinoma cells (LS174T) and INT407 human embryonic intestinal cell line isolated from the jejunum and ileum of a human embryo at about 2 months of gestation were obtained from the ATCC, and cultured in the recommended medium.
NHDF-neo (normal human dermal fibroblasts- neonatal), HMEC (human mammary epithelial cells), and NHP (normal human prostate) cells were purchased from Lonza (Walkersville, MD USA) and cultured in company recommended medium [16], [18], [32], [33], [34], [35].
All the cell lines were grown in the recommended medium supplemented with 10% of fetal bovine serum (Natocor, Cordoba, Argentina), 2 mM glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin and maintained in a 37°C atmosphere containing 5% CO2.
Similar(32)
This procedure, however, is quite cumbersome, and tests were performed for low-energy high-resolution (LEHR) collimator which presents additional challenges (need to account for high scatter and septal penetration) relative to the typically recommended medium-energy (ME) collimator [14, 16, 17].
(He recommends medium rare).
Last January, after seeing sizable run-ups in the larger biotechs, he started recommending medium-sized companies.
The double and triple-layer PA courses are only recommended in medium traffic roads, and the performance in the field need further investigated and validated.
After that, 1 mL (108 CFU/ml) of this seed culture was taken to inoculate 100 mL of recommended agar medium for each indictor strain.
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