Sentence examples for recommended for sequencing from inspiring English sources

This is in broad agreement with the 20 to 40+ clones recommended for sequencing multiple-copy nuclear genes using bacterial cloning [ 13].

Despite its ability to access genuine ancient sequence information, the oligonucleotide spiking approach tested here could not be recommended for sequencing ancient DNA extracts unless further developments are performed in order to optimize the filtering of oligonucleotide sequences and to prevent the shifts observed both in base composition and sequence diversity.

Ion Torrent semiconductor sequencing is not recommended for sequencing of extremely AT-rich genomes, due to the severe coverage bias observed.

Specifically, the G-INS-I strategy was selected using standard settings, as it is recommended for sequences sharing global homology.

Those sequences were first re-aligned using MAFFT v6 [ 60], using the E-INS-i option recommended for sequences with multiple conserved domains and long gaps [ 61].

The cDNA sequence dataset (Additional file 2) was aligned first using MAFFT v6 [ 60], using the G-INS-i option recommended for sequences with global homology [ 61].

Simple randomisation with a 1 1 allocation ratio is analogous to a coin toss, although tossing a coin is not recommended for sequence generation.

This fell within the acceptable manufacturer recommended range for sequencing.

Although the CircLig protocol does involve more hands-on time than the standard Life Technologies protocol, it offers superior accuracy and therefore we recommend it for sequencing on Life Technologies platforms.

We followed the quality control steps recommended for bisulfite sequencing data (69) to ascertain the accuracy of the methylation calls.

The following experiments were performed according to the specific set of mapping parameters recommended for each sequencing platform.