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We used the Qiagen Multiplex PCR Kits following manufacturer recommendations, with the following adjustments (recommended for more than 10 PCR products): our primer concentration was decreased to a final concentration of 0.1 μM and the annealing time was increased to 3 min in a total volume of 10 μl.
And none of these drugs are typically recommended for more than a week at a time.
The American Red Cross has recommended for more than a decade that households include duct tape and plastic sheeting in an emergency safety kit.
But NTSB chairman Christopher Hart warned that unless the technology was put fully in place soon, "I'm very concerned that we're going to be back in this room again, hearing investigators detail how technology that we have recommended for more than 45 years could have prevented yet another fatal rail accident".
The use of drip irrigation is recommended for more effective irrigation without excessive evaporation and also for preventing weathering and leaching.
A participant agrees with the proposal if it has tested the process as recommended for more than ζ/2 consecutive testing rounds.
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If you are short on time, you may cook for as little as one hour, but a cooking time of two or more hours is recommended for a more flavorful stock.
Delayed phase acquisition is recommended for a more accurate estimation of true extent of false lumen thrombus vs. slow flow.
A semi-competitive reserve-type market approach is suitable for an emerging market and a competitive exchange is recommended for a more mature market.
Parental guidance is also recommended for the more complex projects, making for a great opportunity to bond with kids through creation with a side of frustration.
In summary, a minimum daily intake of 300 mg/day of EPA + DHA, of which 200 mg/day are DHA or up to 700 1000 mg of EPA + DHA/day is highly recommended for a more favourable pregnancy and lactation [145].
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ordinance for more
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recommended for strengthening
recommended for measures
recommended for improved
recommended for increasing
recommendation for more
recommended for enhanced
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com