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Based on the estimated models, optimum peening conditions are recommended by using the desirability function approach, which is the most popular technique for optimizing multiple responses.
The reason for this behavior is that the suitable target BS or RS can be recommended by using the proposed algorithm.
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The 12 food groups recommended by FANTA using a 24-hour recall method were also used to assess the dietary diversity score of households [ 15].
RNA quality was assessed by visualisation on a denaturing formaldehyde RNA gel (protocol recommended by Qiagen) using ethidium bromide staining.
Labelling and hybridisation procedure was performed as recommended by manufacturer, using miRCURY LNA microRNA Power Labeling Kit (Exiqon).
Labelling of RNA was done as recommended by Agilent Technologies using the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling.
The detection was further facilitated by the new screening method for detection of beta-lactam resistance in H. influenzae recommended by EUCAST using PcG.
Raw Ct values were calculated as recommended by ABI using the RQ manager software v1.2.1 (ABI) with automatic baseline and threshold settings.
Reverse transcription and subsequent amplification and labelling were carried out in accordance with protocols recommended by Affymetrix using the BioArray HighYield RNA Transcript Labeling kit (ENZO Life Sciences, Farmingdale, NY, USA).
Assay was performed following the procedures recommended by suppliers, using a 96 well flat bottom plates (Corning Inc ,Costar) coated with Taxol-protein antigen.
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