Because so few participants met none of the lifestyle recommendations, we pooled the groups with zero and one point.
Stock UHRR tubes were prepared following manufacturer's recommendation and pooled to create a stock of 1 mg/ml; Brain RNA was received at 1 mg/ml.
Recommendations to pool surgical expertise were also included in a 2007 report by the Royal College of Surgeons and the 2001 report of Professor Sir Ian Kennedy's public inquiry following the tragic deaths of children at Bristol between 1984 and 1995.
Array comparative genomic hybridization was performed using a Roche NimbleGen CGX v1 315K array (Roche NimbleGen, Madison, WI) per manufacturer's recommendations using healthy pooled male and female reference DNA (Promega, Madison, WI), and arrays were analyzed using Genoglyphix software (Signature Genomics, Spokane, WA).
Thus the agency's brochure "Fecal Accident Response Recommendations for Pool Staff: What Do You Do When You Find Poop in the Pool?" and its poster that looks like an eye chart.
For the recommendation for non-smoking, we pooled former smokers and never smokers.
In order to give practical recommendation for LiST tool, we pooled results from studies which provided data on actual mean change in weight (kg) and mean change in length (cm).
A data set of the orthologous genes was obtained from the DPGP resequencing project (http://www.dpgp.org/, last accessed October 22, 2014; Pool et al. 2012) from the Rwandan sample of 17 D. melanogaster haploid genomes, filtered for introgression from European populations based on the recommendations in Pool et al. (2012), as described in Campos et al. (2014).
In this study, swabs were pooled according to EFSA recommendations [ 35], while in the other two studies, ten to 25 individual samples per farm were analyzed.
Two in particular, pooled each by only one of the reviews, influenced the recommendations in opposite directions.
