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Briefly, GBS pellets were suspended in 200 µl of Max Bacterial Enhancement Reagent (Invitrogen), incubated according to the manufacturer's recommendations, mixed with 1 ml of TRIZOL, and disrupted using lysis matrix B (MP Biochemicals).
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Recommendations: The impact of the Programme to date has been mixed, with a number of areas showing substantial positive impact, while others do not.
The α-smooth muscle-actin antibody was mixed with biotinylated anti-mouse Fab fragments using the Animal Research Kit (Dako) and used for staining following the recommendations of the manufacturer.
Denatured RTA linker-biotin conjugate was mixed with 19S RTA linker-biotins transferred to interaconjugatetners followasg UV illumixedion, using the manufacturer's recommendations (Thermo Scientific).
Disappointment mixed with pride.
Sincerity mixed with strategy.
Grief mixed with shock.
Sometimes, mixed with tears.
DREAD was mixed with anger.
Originals mixed with old masters.
Talent mixed with sheer will.
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CEO of Professional Science Editing for Scientists @ prosciediting.com