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Brain samples were examined by TeSeE WB (Bio-Rad) following the manufacturer's recommendations, as previously described [15].
These breakpoints were used on the basis of recommendations as previously described [ 16].
Genotyping was performed using the humanCVD bead array chip (Illumina) following manufacturers recommendations as previously described (17).
Flexible fibroscopic bronchoscopy with bronchoalveolar lavage: FOB and BAL-sampling were performed according to European Respiratory Society recommendations, as previously described [ 12, 13].
BrdU incorporation into proliferating cells was detected by immunocytochemistry according to the manufacturer's recommendations, as previously described (Lambrot et al. 2006; Livera et al. 2000).
IL-6 levels in the serum and IL-2 levels in conditioned medium were quantified using a BD FACS array and Inflammation Kit (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer's recommendations, as previously described [ 17].
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RNA isolation from Arabidopsis plants was performed with the RNA Aqueous-Midi kit (Ambion, Inc; catalog: #1911) following the manufacturer's recommendations with modifications as previously reported [ 27].
It is a requirement not a recommendation, as previously stated, that nurses and midwives set up an NMC Online account in order to revalidate.
The cells were cultured according to the provider's recommendation as previously described [44].
The manipulation procedures followed the manufacturer's recommendation as previously described (Table 2).
Cell proliferation in the samples was assessed using the Cell Proliferation Reagent WST-1 with OD450 nm proportional to the cell numbers according to the manufacturer's recommendations and as previously described (Cucchiarini et al. 2011; Venkatesan et al. 2012).
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