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A total of 500 ng of DNA-free RNA from each sample was used to synthesize cDNA according to manufacturer's recommendation with random primers (EasyScript cDNA Synthesis Kit, Lamda Biotech, USA).

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First strand synthesis was performed with 5 μg total RNA and the Superscript II reverse transcriptase (Invitrogen) primed with random hexamer oligodeoxynucleotides according to the recommendations of the supplier.

For cDNA synthesis, 200 ng RNA were reverse transcribed using SuperScript™ First-Strand Synthesis System with random hexamers (Invitrogen) according to manufacturer's recommendations.

From each condition, 5  μg of total RNA was reverse transcribed using the SuperScript III First-Strand synthesis system (Invitrogen, Paisley, UK) with random hexamer primers according to manufacturer's recommendations.

cDNA was synthesized by using the ImProm-II Reverse Transcription System (Promega, Madison, WI, USA) with random hexamers as primers according to the manufacturer's recommendation (First BASE Laboratories, Selangor Darul Ehsan, Malaysia).

In brief, 1.0 μg RNA of the diagnostic AML samples was transcribed into cDNA using the SuperScript III Kit with random hexamer primers according to the manufacturer's recommendations (Invitrogen, Carlsbad, CA).

First strand cDNA was synthesized from 1 5 µg total RNA with random primers using SuperScript Reverse Transcriptase III (Invitrogen) according to the manufacturer's recommendations.

Total mRNA was extracted from HeLa cells grown in a T25 flask (Sigma Aldrich) with RNeasy Plus Mini kit (Qiagen) and cDNA was generated with random hexamer primers by reverse transcription (Superscript III, Life Technologies) according to the manufacturer's recommendations.

We found the least agreement (41%) with the recommendation to use random blood (plasma) glucose in the diagnosis of diabetes in patients with classic diabetes symptoms.

First strand cDNA has been synthesized with random hexameres using the High-Capacity cDNA Reverse Transcription Kit according to the manufacture's recommendations (Applied Biosystems).

5. mixtures with random coils.

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