Sentence examples for recombination step from inspiring English sources

Distinct mutations identified as significant for stability were combined by a rational recombination step and the resulting recombinant was further evolved by an additional random mutagenesis round.

Additionally, the method allows the consecutiev modification of recombinant viruses without the need for additional selectable markers at each recombination step.

c λ Red recombination step.

This is followed by a homologous recombination step to provide the genetic information needed to complete repair.

This determination of the different parameters allows us to corroborate and to complete the results obtained by means of pulse methods, and to demonstrate that, in the case of an anodic pretreatment of the electrode, the hydrogen evolution reaction includes an ion + atom recombination step.

This controlled for any change in fitness due to the recombination step.

The ssDNA is then coated with Replication Protein A (RPA) to protect it from nuclease attacks as well as mediate the subsequent recombination steps [18].

Is IntI1 sufficient as sole bacterial protein for performing all the recombination steps or does it require other factors?

The number of recombination steps required to obtain certain arrangement types may also limit which arrangement types naturally occur.

Recently, we presented a method able to generate scarless deletions which is based on two successive λ Red recombination steps [9].

Recombination activity detected in the presence of two attC sites suggests that the initial low binding of the enzyme to the site is sufficient to trigger all the subsequent recombination steps (way C).