We set out to compare different homologous recombination templates and resulting apparent recombinants, with the goal of being able to recommend preferred strategies.
Many natural DNA site-specific recombination systems achieve directionality and/or selectivity by making recombinants with a specific DNA topology.
Domain recombination also led to changes in mating phenotype, including recombinants with increased mating efficiency over the wild type.
Growth phenotype of two CMV recombinants with mutations in UL54 was studied.
The strategy of simultaneous foreground and phenotypic selection to introduce multiple R genes is very useful to reduce the cost and the time required for the isolation of desirable recombinants with target resistance genes in rice.
Although the use of shorter donor end regions decreases total recombinants by several fold, the frequency of recombinants with correctly inserted donor molecules was high enough for easy detection by simple polymerase chain reaction (PCR) screening.
The genomic DNA of every generation was extracted from the 4-day-old colonies according to a documented method (Raeder and Rroda 1985) and used as PCR templates to detect the AaIT gene presence in the recombinants with SP-AaIT-F and SP-AaIT-R primers (5′-CCCAAGCTTATGCGTGAACTTTCTTCGGT-3′ and 5′-CCCTCGAGTTAGTTGATGATGGTGGTAT-3′, respectively).
Both dementia patients contained numerous intra-tissue recombinants with many sequences containing multiple breakpoints along gp120.
Thus, here we present a modification of this method allowing much easier and efficient selection of recombinants with the help of I-SceI-induced double-strand breaks (DSB).
In summary, the six sequenced regions are well separated along Chromosome 4 and span a range of less than 1% to over 45% recombinants with the centromere.
The entire VP1 coding sequences of 10 poliovirus isolates revealed their uncommon genomic intertypic (type 3/type 2) recombinants with crossover sites within the VP1 capsid-coding region.
