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The recombinants were identified by applying recombination detection tests implemented in RDP4 (Martin et al. 2010) to an alignment of the sequences obtained for all six genome components (Savory and Ramakrishnan 2014).
Recombinants were identified by PCR and sequenced.
Recombinants were identified from 1,447 F2 glabrous plants.
Ten informative homozygous recombinants were identified by genotyping F3 individuals with the five markers.
The plaques were screened with DNA containing four copies of the octamer sequence and positive phage recombinants were identified.
Recombinants were identified by restriction analysis of plasmid DNA with Bam H R- Eco R I, Sac I, Nco I and Nde I and were confirmed by sequencing of both the strands.
Similar(10)
CRF28_BF and CRF29_BF are among the first two BF recombinants being identified in Brazil and they contributed significantly to the epidemic.
A relatively high frequency of NoV intergenotype recombinants was identified.
Nevertheless, future typing assays should analyze both VP1/2 and NS/NP-1 to ensure that this and potentially other interspecies recombinants are identified.
Using these techniques, a population of putative inter-lineage recombinants is identified inhabiting the reef building coral Pocillopora damicornis at the isolated, high-latitude reef of Lord Howe Island, Australia.
Among the 324 C/D recombinant isolates, two types of C/D recombinant were identified: 244 belonged to 'CD1' and 80 belonged to 'CD2'CD2
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