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In this study, we demonstrated the broad HIV-1 genetic diversity in Hong Kong and it is the first comprehensive study in Asia revealing the transmission of different new recombinants in the same locality.
Six of 10 samples judged to be methylation-positive on MSP exhibited methylation in nearly all eight recombinants in the same region.
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Semi-quantitative analysis was performed using a series of dilution of recombinant profilin in the same gel.
Surprisingly, the basal level of auto-phosphorylation is quite low compared to that we could expect for a recombinant IR in the same conditions [43].
The resulting trees put the recombinant sequences in the same positions as those inferred by cBrother.
The response to a specific race in the XC population was evaluated by inoculating all recombinant lines in the same test, including 8 10 seedlings per line.
Primers were listed in Table 3.> Cells were plated and infected with recombinant adenoviruses in the same manner as that for qPCR.
Then, Huh-7 cells were treated with 5, 10, 20, and 40 μg/mL of each recombinant protein in the same medium for 2 hours.
The combination of different techniques, like immunochromatographic test (ICT) and rapid immunoenzyme assay, or the employment of different antigenic targets and the combination of distinct recombinant proteins in the same assay are strategies that may improve the global specificity and sensitivity, thus bringing a higher accuracy to the routine protocols [ 8– 10].
In order to get more insights about the role of MNT2 and MNT5; we heterologously expressed them in P. pastoris, characterized the recombinant enzyme activities, and compared them with recombinant Mnt1 obtained in the same expression system.
The FLAG-GST tag and recombinant glucagon were in the same sample to in vivo biological activity, but not were fusioned.
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