The demands for recombinant proteins, in addition to plasmid DNA, for therapeutic use are steadily increasing.
It is noted that this group also confirmed the direct interactions between PGRN and TNFR in a solid phase binding assays with recombinant proteins, in addition to the in vivo interactions, as assayed by Co-IP.
However, the criteria for selecting the most suitable animal species for molecular pharming are based on the quantity of proteins needed per year, the capacity of a facility, and the potential commercial value of the recombinant proteins in addition to other factors such as time until milk production and milk volume.
Analysis of the substrate range revealed that the recombinant enzyme catalyzes, in addition to the conversion of some classic peroxidase substrates such as 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate
PBMCs were cultured with or without recombinant human C5a in addition to P. falciparum culture supernatants (1∶10 dilution) and R10G alone as media control, or HPLC-purified GPI (300 ng/mL) and unconjugated gold beads as a control.
This subpopulation of distal tubular cells was also able to internalize recombinant apical CTGF, in addition to proximal cells which were the main cells to take up exogenous CTGF.
Moreover, since all CENH3-GFP harboring plants do not show any physiological or developmental defect and display a growing behavior similar to wild type, we conclude that the introgression of recombinant CENH3 proteins, in addition to the endogenous CENH3 protein, does not interfere with the functional role of the centromere in mitotic and meiotic cell division.
The level of isoprene production for B. subtilis and B. licheniformis harboring the recombinant plasmid pHT01-kIspS, in addition to E. coli BL21 DE3) harboring the recombinant plasmid pET28b-kIspS were investigated using different IPTG concentrations (i.e., 0.1, 0.5, 1 and 2 mM) for 4 h incubation at the suitable temperature for each.
Figure 4a shows a summary of the helical fragments which retained solubility after recombinant expression and purification, in addition to two shorter synthesized peptides.
