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The present work demonstrated for the first time that, like intersubtype recombinants that have caused epidemics, such as CRF02_AG, CRF07_BC, CRF08_BC, and BF [ 31– 34], intrasubtype recombinants from the same recombination event can also lead to the prevalence of HIV-1 and AIDS.
Such clustering in geographic distribution and the fact that distinct CRF01_AE lineages were independently introduced to China from Southeast Asia suggest that these intrasubtype recombinants very likely originated within this region and that there have probably been epidemics caused by intrasubtype recombinants from the same recombination event.
The removal of all recombinants from the data set eventually increased the PHI-test p-value to a level of no significance.
In order to test the impact of an inactivation of mutY on length of import and ISR, a prescreening procedure was applied to select recombinants from the Rif resistant clones isolated from the transformation experiment.
These 510 susceptible F2 plants, together with 28 recombinants from the unselected F2 mapping population, formed the F2 sub-population for fine-mapping of Scmv1.
To identify genes associated with this phenotype, we are currently establishing more recombinants from the congenic strains carrying the shorter chromosomal segments.
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We confirmed the result in 2005 and obtained one useful recombinant from the cross between Kinmaze and IR24.
Simulations were also performed using the observed frequency of wild-type non-recombinants from the sum across replicates as the expected frequency of wild-type non-recombinants for each replicate in the simulation.
After genotyping, 88 recombinants derived from the CML277 introgressed NIL and 93 recombinants derived from the CML333 introgressed NIL were recovered.
Recombination breakpoints for the E1-E2 region were mainly located in the conserved region between hypervariable regions 1 (HVR1) and 3 (HVR3), and the recombinant fragments from the double recombinant sequences detected spanned the entire HVR1 or HVR3 regions.
Previously we documented proliferation of at least five recombinants derived from the A and B variants of the CR1-Zm subfamily and one recombinant derived from A and B variants of the CR4-Zm subfamily (Sharma et al. 2008).
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