Sentence examples for recombinant expression vector from inspiring English sources

As shown in Figures 6a c, primary transcripts of the human (a), rat (b), or dog (c) miR-433 and miR-127 were easily detected in cells that over-expressed the recombinant expression vector, respectively.

In this way, recombinant expression vector tan-pET43.1a was formed.

This technical innovation of pOmni makes it possible to construct a recombinant expression vector in 8 h by starting with the PCR amplification of the target gene.

Almost 1 week is necessary even for an experienced researcher to construct recombinant expression vector of a target gene by means of above cloning strategy.

The recombinant expression vector plasmid pPG612-PLFN was created and identified by using plasmid extraction, PCR, restriction enzyme digestion and sequence analysis.

In order to overcome above mentioned inconvenience of constructing recombinant expression vector of a target gene, we elaborately designed and constructed an omnipotent expression vector designated as pOmni that has two advantageous technical features.

The recombinant expression vectors were transformed into E. coli BL21 DE3).

The recombinant expression vectors were then linearized with Sac I and introduced into P. pastoris strain GS115.

The approach of calcium phosphate transfection was used to transfect two recombinant expression vectors above and empty vector pIRES2-EGFP (1 μg/1μL) into 293FT cell (laboratory storage).

To assess possible localization of TCD10, we constructed the recombinant expression vectors pMON530-GFP-TCD10 and transformed into tobacco cells by Agrobacterium-mediated infection.

DNA sequence analysis of the five recombinant expression vectors pPICZα-HSA-UTI demonstrated that cDNA encoding human bikunin and human serum albumin (HSA) gene were correctly inserted into pPICZαA vector.