Sentence examples for recombinant expression strain from inspiring English sources

E. coli strain DH5α (Novagen, Madison, USA) was employed for gene manipulation and BL21 (DE3; Novagen, Madison, USA) was employed to construct recombinant expression strain.

Lastly, the pDEP plasmid can then be transferred into a bacterial expression strain for recombinant protein expression in a prokaryotic system.

Two P. pastoris GS115 his− (Cregg et al. 1993 -derived recombinant strains, the high β-galactosidase expression strain P. pastoris G1993 -derivedow exprecombinanttrol strainsP. pastheis Ghighwere constructed through changing the initiation strength of the constitutive promoter pGAP (Qin et al. 2011).

(3) Already in 1995, Hesse et al. utilized non-pathogenic E. coli strains for the recombinant expression of clostridial collagenases, exploiting the short generation times, easy handling and the well established fermentation know-how of this host, resulting in expression yields several fold higher than in the natural host (Hesse et al. 1995).

Recombinant Ssh10b protein was expressed in the Escherichia coli BL21 (Dexpressionstraintrand and purified as described previously [9].

One study tested the recombinant expression of yeast TM proteins in a yeast host strain.

In order to monitor Cre recombinant expression in vivo, it is important to create a reporter strain.

In brief, E. coli Rosetta (DE3) strain cells (Novagen, Germany) were transformed with recombinant expression vector pET21a-CA XII.

The robotic procedures for high-throughput growth of bacterial expression strains, recombinant subunit purification and automated assembly into recombinant RNAPs (the 'RNAP Factory') have been described previously [ 19, 20, 58].

The final recombinant expression vector pET26b, containing the DI gene and a kanamycin resistance gene, was transferred to the expression strain BL21 DE3).

The recombinant expression vector was transformed into Pichia pastoris GS115, and the recombinant strains were screened by SDS-PAGE.