Sentence examples for recombinant expression purification from inspiring English sources
Although gene sequence was highly similar to other B. velezensis stain JS25R, this study is the first report of the recombinant expression, purification, and characterization of the enzyme.
In this study, we report on the recombinant expression, purification, and characterization of CYP154H1, a new moderately thermostable bacterial P450 monooxygenase from T. fusca.
In the present paper, we report investigations into the first steps of both branches of the Kennedy pathway, involving molecular cloning, recombinant expression, purification and detailed kinetic characterization of both ethanolamine and choline kinases (EK and CK respectively) from T. brucei.
Recombinant expression and purification of LFn-FlaA was performed as previously described.
On the basis of such findings, this work deals with the recombinant expression and purification of Poly s 5 in Pichia pastoris.
The following article describes an optimized procedure for recombinant expression and purification of PglB, a bacterial OTase, in a stably active form.
To facilitate renewed efforts in CPG2 structural biology, we report the first high-yield (250 mg L−1) recombinant expression (and purification) of soluble and active CPG2 using the Escherichia coli expression system.
Herein, we present strategies for the development of a high-throughput thermal denaturation-based assay for identifying STAT inhibitors as well as high-yielding recombinant expression and purification of untagged STAT1, STAT3, and STAT5 proteins.
Upon construction of the synthetic gene, recombinant expression and purification, the first phytase obtained, termed consensus phytase-1, displayed an unfolding temperature (Tm) of 78.0°C which is 15 22°C higher than the Tm values of all parent phytases used in its design.
In this work, recombinant expression and purification of 2,5-DKCMO, an oxygenating subunit, led to a "dehydrogenase-missing" pure protein and it could be shown that the enzyme is still able to oxidize bicyclic ketones.
Figure 4a shows a summary of the helical fragments which retained solubility after recombinant expression and purification, in addition to two shorter synthesized peptides.
