Exact(1)
TaqI recognizes the restriction site TCGA and cleaves the resistance allele but not the susceptible allele due to the alteration of the restriction site (i.e., TCGG) by the SNP.
Similar(59)
Even if the expected mean squares recognize the restrictions on randomization, the usual F test will not give predictable results because its numerator and denominator are correlated.
Using two isoschizomer restriction enzymes (MspI and HpaII), which recognize the same restriction site (5-CCGG-3) but have different cytosine methylation sensitivities, MSAP allows the identification of CpG-methylation polymorphism [6], [23] [25].
MfAFLP is a modified version of standard AFLP [ 13] using a pair of methylation-sensitive restriction enzymes, H pa II and M sp I, which are a pair of isoschizomers, recognize the same restriction site CCGG but possess differential methylation sensitivity at the inner or outer cytosine [ 14].
For each contig, SOMA2 computationally recognizes all the restriction sites and cleaves the contig into an ordered set of RFs.
Since the Rule is just as silent on the subject of seizing intangibles as it was on the subject of seizing mere evidence, it is difficult to understand why the Court does not recognize the same "restriction" against such seizures.
These enzymes recognize the same restriction site (CCGG) but have different sensitivities to certain methylation states of cytosines.
HpaIIand MspI form a pair of isoschizomers that recognize the same restriction site (5'-CCGG) but have different sensitivity to methylation of the cytosines.
HpaII and MspI are a pair of isoschizomers that recognize the same restriction site (5'-CCGG) but have differential sensitivity to certain methylation states of the two cytosines: HpaII will not cut if either of the cytosines is fully (double-strand) methylated, whereas MspI will not cut if the external cytosine is fully- or hemi- (single-strand) methylated [ 40].
Cytosine methylation changes can be detected using methylation-sensitive amplification polymorphism (MSAP), an AFLP method that uses a pair of isoschizomers, MspI and HpaII [ 23] that recognize the same restriction site (CCGG) but have different sensitivity to certain methylation states of cytosines.
The potentially lethal cleavage of cellular DNA in cells that harbour a restriction enzyme is prevented by epigenetic DNA methylation by the cognate DNA methyltransferase that recognizes the same sequence as the restriction enzyme (Fig. 1A and B).
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